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An Introduction to the Theory and Practice of LC Method Translations and LC Method Transfers |
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In this recorded webinar, we focus on isocratic and gradient method translations. This includes adjusting column dimensions (e.g., scaling a method up or down); moving between different instruments (e.g., transferring a method between sites); and changing particle morphologies (e.g., between fully porous and solid-core particles). We introduce the theory behind such translations and illustrate each situation with several practical examples, including presenting data from successful translations and providing common reasons for failed method translations. Finally, we discuss how to use the free ACE® method translation tool* for automatically performing calculations. |
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*ACE Excel tool can also help you do other very useful calculations (measuring gradient delay volume, extra-column volume, column porosity, mobile phase consumption from isocratic and gradient runs, column volume, and column equilibration volume) |
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What You'll Learn About: |
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Method translation theory
- Moving methods from HPLC to UHPLC and vice versa
- Instrument-to-instrument method transfer
- Tips and tricks for translating methods
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